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Inhalation versus intraperitoneal oxytocin administration in mice

DISCOVERIES REPORTS (ISSN 2393249X), 2016, volume 2

CITATION: 

Kobylinska L, Geagulea G, Berbecar V, Stancu M, Stefan AM, Zagrean AM, Zagrean L. Inhalation versus intraperitoneal oxytocin administration in Swiss-Albino Mice. Discover. Rep. 2016; 2: e5. DOI: 10.15190/drep.2016.1

 Submitted: May 20th, 2016; Revised: June 10th, 2016; Accepted: June 22nd, 2016Published: July 3rd, 2016;

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Inhalation versus intraperitoneal oxytocin administration in Swiss-Albino Mice 

Liana Kobylinska (1,2,*), Gabriel Geagulea (3), Vlad Berbecar (4), Mihai Stancu (3), Adriana Maria Stefan (3), Ana-Maria Zagrean (2), Leon Zagrean (2,*) 

(1) Child and Adolescent Psychiatry Department, “Prof. Dr. Alexandru Obregia” Clinical Psychiatry Hospital, Bucharest, Romania;

(2) Physiology and Fundamental Neuroscience Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania;

(3) Romanian Group of Research in Neuroscience, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania;

(4) Nephrology Department, Fundeni Clinical Institute, Bucharest, Romania;

*Correspondence toLeon Zagrean, MD, PhD, Head of the Department of Physiology, Carol Davila University of Medicine and Pharmacy and Liana Kobylinska, MD, “Prof. Dr. Al. Obregia” Clinical Psychiatry Hospital, Bucharest, Romania; Emails: lzagrean@univermed-cdgm.ro and vnedescu@yahoo.com

Abstract

BACKGROUND: Oxytocin modulates several social behaviors, with research results focusing on its role as potential adjunctive dedication in psychiatric disorders. This raises the necessity for a less stressful, translational way of oxytocin delivery in animal models. The aim of this paper is to provide a comparison between mice’s open field test (OFT) behavior after a single inhalator (Inh), respectively intraperitoneal (IP) oxytocin administration.

METHODS: Forty-four male Swiss-Albino mice were divided into three groups- oxytocin, no-procedure (control) and placebo, according to the administered substance. The substances were delivered either through IP injection, or through a 22 minutes nebulization. The mice in the oxytocin group received 1 UI of oxytocin, whereas those in the placebo group got the equivalent amount of saline. OFT was performed 30 to 90 minutes after the administration.

RESULTS: The time spent in the center varied among the groups (F(4,44)=3.224, p=0.022), due to the fact that the mice that received inhalatory oxytocin spent more time in the center than those in the control group and than those that were administered the substances intraperitoneally. The mice that received IP oxytocin had fewer boli emissions than all the other mice, whereas the mice that received Inh oxytocin had more boli emissions that those that received placebo, either inhalatory or intraperitoneally.

CONCLUSION: We may conclude that group inhalator delivery of oxytocin seems preferable to the intraperitoneal injection way, with higher anxiolytic effects at the same dose.

 

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References

1. Dumais KM, Veenema AH. Vasopressin and oxytocin receptor systems in the brain: Sex differences and sex-specific regulation of social behavior. Front Neuroendocrinol. 2015; S0091-3022:27-28

2. Uzefovsky F, Shalev I, Israel S, Edelman S, Raz Y, Mankuta D et al.Oxytocin receptor and vasopressin receptor 1a genes are respectively associated with emotional and cognitive empathy. Horm Behav. 2015; 67:60-65

3. Saphire-Bernstein S, Way BM, Kim HS, Sherman DK, Taylor SE. Oxytocin receptor gene (OXTR) is related to psychological resources. Proc Natl Acad Sci U S A. 2011; 108:15118-15122

4. Lucht MJ, Barnow S, Sonnenfeld C, Rosenberger A, Grabe HJ, Schroeder W et al. Associations between the oxytocin receptor gene (OXTR) and affect, loneliness and intelligence in normal subjects. Prog Neuropsychopharmacol Biol Psychiatry. 2009; 33:860-866

5. Kim HS, Sherman DK, Sasaki JY, Xu J, Chu TQ, Ryu C et al. Culture, distress, and oxytocin receptor polymorphism (OXTR) interact to influence emotional support seeking. Proc Natl Acad Sci U S A. 2010; 107:15717-15721 

6. Gallup AC, Church AM. The effects of intranasal oxytocin on contagious yawning. Neurosci Lett. 2015; 607:13-16

7. Huang H, Michetti C, Busnelli M, Managò F, Sannino S, Scheggia D et al. Chronic and acute intranasal oxytocin produce divergent social effects in mice. Neuropsychopharmacology. 2014; 39:1102-1114

8. Windle RJ, Shanks N, Lightman SL, Ingram CD. Central oxytocin administration reduces stress-induced corticosterone release and anxiety behavior in rats. Endocrinology. 1997; 138:2829-2834

9. Baumgartner T, Heinrichs M, Vonlanthen A, Fischbacher U, Fehr E. Oxytocin shapes the neural circuitry of trust and trust adaptation in humans. Neuron. 2008; 58:639-650

10. Zak PJ, Stanton AA, Ahmadi S. Oxytocin increases generosity in humans. PLoS One. 2007; 2:e1128.

11. Kosfeld M, Heinrichs M, Zak PJ, Fischbacher U, Fehr E. Oxytocin increases trust in humans. Nature. 2005; 435:673-676.

12. Marsh AA, Yu HH, Pine DS, Blair RJ. Oxytocin improves specific recognition of positive facial expressions. Psychopharmacology (Berl). 2010; 209:225-232.

13. Shalvi S, De Dreu CK. Oxytocin promotes group-serving dishonesty. Proc Natl Acad Sci U S A. 2014; 111:5503-5507

14. Neumann ID, Slattery DA. Oxytocin in General Anxiety and Social Fear: A Translational Approach. Biol Psychiatry. 2016; 79:213-221

15. Harrison AJ, Gamsiz ED, Berkowitz IC, Nagpal S, Jerskey BA. Genetic variation in the oxytocin receptor gene is associated with a social phenotype in autism spectrum disorders. Am J Med Genet B Neuropsychiatr Genet. 2015; 168:720-729. 

16. Rich ME, Caldwell HK. A Role for Oxytocin in the Etiology and Treatment of Schizophrenia. Front Endocrinol (Lausanne). 2015; 6:90.

17. Bartholomeusz CF, Ganella EP, Labuschagne I, Bousman C, Pantelis C. Effects of oxytocin and genetic variants on brain and behaviour: Implications for treatment in schizophrenia. Schizophr Res. 2015.

18. Chagnon YC, Potvin O, Hudon C, Préville M. DNA methylation and single nucleotide variants in the brain-derived neurotrophic factor (BDNF) and oxytocin receptor (OXTR) genes are associated with anxiety/depression in older women. Front Genet. 2015; 6:230.

19. Mercedes Perez-Rodriguez M, Mahon K, Russo M, Ungar AK, Burdick KE. Oxytocin and social cognition in affective and psychotic disorders. Eur Neuropsychopharmacol. 2015; 25:265-282.

20.  Kirsch P. Oxytocin in the socioemotional brain: implications for psychiatric disorders. Dialogues Clin Neurosci. 2015; 17:463-476

21. Hammen C, Bower JE, Cole SW. Oxytocin receptor gene variation and differential susceptibility to family environment in predicting youth borderline symptoms. J Pers Disord. 2015; 29:177-192

22. Kent P, Awadia A, Zhao L, Ensan D, Silva D, Cayer C et al. Effects of intranasal and peripheral oxytocin or gastrin-releasing peptide administration on social interaction and corticosterone levels in rats. Psychoneuroendocrinology. 2016; 64:123-130

23. Heredia L, Torrente M, Domingo JL, Colomina MT. Individual housing and handling procedures modify anxiety levels of Tg2576 mice assessed in the zero maze test. Physiol Behav. 2012; 107:187-91

24. Novak J, Bailoo JD, Melotti L, Rommen J, Würbel H. An Exploration Based Cognitive Bias Test for Mice: Effects of Handling Method and Stereotypic Behaviour. PLoS One. 2015; 10:e0130718.

25. http://www.informatics.jax.org/mgihome /other / mouse _facts1.shtml

26. Crawley JN. Exploratory behavior models of anxiety in mice. Neurosci Biobehav Rev. 1985; 9:37-44

27. Ring RH, Malberg JE, Potestio L, Ping J, Boikess S, Luo Bet al. Anxiolytic-like activity of oxytocin in male mice: behavioral and autonomic evidence, therapeutic implications. Psychopharmacology (Berl). 2006; 185:218-225.

28. Crine AF, Boulanger B, Nizet G. Effect of daily pretrial injection of oxytocin on rat behavior in the open-field situation. Regul Pept. 1983; 5:145-152.

29. Pedersen CA, Vadlamudi S, Boccia ML, Moy SS. Variations in Maternal Behavior in C57BL/6J Mice: Behavioral Comparisons between Adult Offspring of High and Low Pup-Licking Mothers. Front Psychiatry. 2011; 2:42.

30. Bales KL, Perkeybile AM, Conley OG, Lee MH, Guoynes CD, Downing GM, et al.: Chronic intranasal oxytocin causes long-term impairments in partner preference formation in male prairie voles. Biol Psychiatry. 2013; 74:180–188

31. Bales KL, Solomon M, Jacob S, Crawley JN, Silverman JL, Larke RHet al. Long-term exposure to intranasal oxytocin in a mouse autism model. Transl Psychiatry. 2014; 4:e480.

32. Neumann ID, Maloumby R, Beiderbeck DI, Lukas M, Landgraf R. Increased brain and plasma oxytocin after nasal and peripheral administration in rats and mice. Psychoneuroendocrinology. 2013; 38:1985-1993.

33. Bale TL, Davis AM, Auger AP, Dorsa DM, McCarthy MM. CNS region-specific oxytocin receptor expression: importance in regulation of anxiety and sex behavior. J Neurosci. 2001; 21:2546-2552.

34. Bülbül M, Babygirija R, Ludwig K, Takahashi T. Central oxytocin attenuates augmented gastric postprandial motility induced by restraint stress in rats. Neurosci Lett. 2010;479:302-306. 

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