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RCI-Oncogene viruses for tumor specific knockout

DISCOVERIES (ISSN 2359-7232), 2016, April-June issue

CITATION: 

Wang W, Dong B, Ittmann MM, Yang F. A Versatile Gene Delivery System for Efficient and Tumor Specific Gene Manipulation in vivo. Discoveries 2016, April-June; 4(2): e58. DOI: 10.15190/d.2016.5

Submitted: June 10, 2016; Revised: June 21, 2016; Accepted: June 21, 2016; Published: June 22, 2016;

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A Versatile Gene Delivery System for Efficient and Tumor Specific Gene Manipulation in vivo

Wei Wang (1), Bingning Dong (1), Michael M. Ittmann (2), Feng Yang (1,*)

(1) Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA

(2) Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, 77030, USA

*Correspondence to: Feng Yang, Ph.D., Department of Molecular and Cellular Biology, One Baylor Plaza, Baylor College of Medicine, Houston, TX, 77030, USA; Email: fyang@bcm.edu

Abstract

The Replication-Competent Avian Sarcoma-leukosis virus long-terminal repeat with splice acceptor (RCAS)-Tumor Virus A (TVA) gene delivery system has been created based on the fact that avian sarcoma leukosis virus subgroup A only infects cells expressing its receptor, TVA. This system has been successfully applied to create various mouse models for human cancers. Here we briefly discuss the advantages and the potential caveats of using this RCAS-TVA gene delivery system in cancer research. We also introduce and discuss how our newly designed RCAS-based gene delivery system (RCI-Oncogene, for RCAS-Cre-IRES-Oncogene) allows concise and efficient manipulation of gene expression in tumors in vivo, and how this system can be used to rapidly study the biological function of gene(s) and/or the collaborative actions of multiple genes in regulating tumor initiation, progression and/or metastasis. 

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