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Nanoscale imaging of E. coli cells by expansion microscopy

DISCOVERIES (ISSN 2359-7232), 2019, July-September issue

CITATION: 

Cheng S, Zhao Y. Nanoscale imaging of E. coli cells by expansion microscopy. Discoveries 2019, 7(3): e98. DOI: 10.15190/d.2019.11

Submitted: September 27th, 2019; Revised: September 29th, 2019; Accepted: September 29th, 2019; Published: September 30th, 2019; 

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Nanoscale imaging of E. coli cells by expansion microscopy

Sharey Cheng, Yongxin Zhao (*)

Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA

*Corresponding author: Yongxin Zhao, PhD, Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA, Phone: +1 412-268-8236; Email:yongxinz@andrew.cmu.edu

Abstract

Expansion microscopy (ExM) is an emerging super-resolution imaging technology. ExM works by infusing a biological specimen with a superabsorbent hydrogel, followed by mechanical homogenization and isotropical expansion of the specimen in water. The unique and cost-effective process of ExM enables super-resolution optical imaging of sample of interest without the need to invest and use of a sophisticated microscope instrument. Here, we demonstrate that a nearly 3-fold isotropic physical expansion of E.coli fixed cells can be achieved in PBS, and the cell morphology during binary fission is clearly resolved in the expanded state, using a diffraction-limited microscope.


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References

1. Wassie AT, Zhao Y, Boyden ES. Expansion microscopy: principles and uses in biological research. Nat Methods. 2019; 16(1):33-41. doi:10.1038/s41592-018-0219-4

2. Chen F, Tillberg PW, Boyden ES. Expansion Micrsocopy. Science.2015 Jan 30; 347(6221):543-8. doi:10.1126/science.1260088

3. Tillberg PW, Chen F, Piatkevich KD, et al. Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol. 2016; 34(9):987-992. doi:10.1038/nbt.3625.

4. Zhao Y, Bucur O, Irshad H, et al. Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy. Nat Biotech. 2017; 35(8):757-764. doi: 10.1038/nbt.3892.

5. Costerton JW, Ingram JM, Cheng KJ. Structure and function of the cell envelope of gram negative bacteria. Bacteriol Rev. 1974.

6. Zhang YS, Chang J-B, Alvarez MM, et al. Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications. Sci Rep. 2016;6(1):22691. doi:10.1038/srep22691

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