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Homocysteine in Multiple Sclerosis

DISCOVERIES (ISSN 2359-7232), 2021, July-September issue

CITATION: 

Mititelu RR, Albu CV, Bacanoiu MV, Padureanu V, Padureanu R, Olaru G, Buga AM, Balasoiu M. Homocysteine as a Predictor Tool in Multiple Sclerosis. Discoveries 2021, 9(3): e135. DOI: 10.15190/d.2021.14


Submitted: July 28, 2021; Revised: September 20, 2021; Accepted: September 27, 2021; Published: September 28, 2021; 

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Homocysteine as a Predictor Tool in Multiple Sclerosis

Radu Razvan Mititelu (1, #), Carmen Valeria Albu (2, #), Manuela Violeta Bacanoiu (4, #), Vlad Padureanu (3, #), Rodica Padureanu (3), Gabriela Olaru (4), Ana-Maria Buga (5,*), Maria Balasoiu (1)

(1) Department of Microbiology, University of Medicine and Pharmacy of Craiova, Romania

(2) Department of Neurology, University of Medicine and Pharmacy of Craiova, Romania

(3) Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, Romania 

(4) Department of Sports and Kinetic Therapy, University of Craiova, Romania

(5) Department of Biochemistry, University of Medicine and Pharmacy of Craiova, Romania


*Corresponding authors: Ana Maria Buga, Department of Biochemistry, University of Medicine and Pharmacy of Craiova, 200349, Romania; Email: anamaria.buga@umfcv.ro; Phone: +40-0351-443 500

 # These authors contributed equally to this work

Abstract

Multiple sclerosis (MS) is a progressive and irreversible disease which affects the central nervous system (CNS) with still unknown etiology. Our study aimes to establish the homocysteine pattern that can predict the MS diseases progression and to identify a potential disease progression marker that can be easy to perform and non-invasive, in order to predict the diseases outcome. In order to achieve this goal, we included 10 adult RRMS subjects, 10 adult SPMS subjects and 10 age-matched healthy subjects. The homocysteine plasma level was measured using automated latex enhanced immunoassay and the cobalamin and folate measurements were performed using automated chemiluminescence immunoassay (CLIA). HCR was calculated by dividing the homocysteine plasma level by cobalamin plasma level. We found that the homocysteine level in plasma of both RRMS patients and SPMS group are significantly increased compared with the control group. There is a significantly higher concentration of homocysteine in SPMS group compared with the RRMS group. In addition, the HCR is significantly increased in SPMS compared with the RRMS group and is a very good index of disease severity.

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